Microarray:Article Title: Gene Expression Profiling of the Hypoxia Signaling Pathway in Hypoxia-Inducible Factor 1α Null Mouse Embryonic Fibroblasts
Article Snippet: The full 15K set was printed on TelechemTM Superaldehyde slides (Telechem International Inc., Sunnyvale, CA) using an OmniGridTM robot (GenMachines ® , San Carlos, CA) with Chipmaker 2 microarray pins (Telechem International Inc). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Gene Accession # Forward Reverse M13 CTGCAAGGCGATTAAGTTGGGTAAC CTGCAAGGCGATTAAGTTGGGTAAC HPRT {"type":"entrez-nucleotide","attrs":{"text":"NM_013556","term_id":"96975137","term_text":"NM_013556"}} NM_013556 AAGCCTAAGATGAGCGCAAG TTACTAGGCAGATGGCCACA GAPDH {"type":"entrez-nucleotide","attrs":{"text":"M32599","term_id":"193423","term_text":"M32599"}} M32599 ACCCAGAAGACTGTGGATGG TTCAGCTCTGGGATGACCTT P4H {"type":"entrez-nucleotide","attrs":{"text":"NM_011031","term_id":"226874875","term_text":"NM_011031"}} NM_011031 CTGTTGTGGCCCGAGTAAAT GAAGTCGAAGTGCGGTTCAT PHD1 {"type":"entrez-nucleotide","attrs":{"text":"AF453879","term_id":"18182545","term_text":"AF453879"}} AF453879 GGAACCCACATGAGGTGAAG AACACCTTTCTGTCCCGATG PHD2 {"type":"entrez-nucleotide","attrs":{"text":"XM_134453","term_id":"25071364","term_text":"XM_134453"}} XM_134453 GAAGCTGGGCAACTACAGGA CATGTCACGCATCTTCCATC PHD3 {"type":"entrez-nucleotide","attrs":{"text":"NM_028133","term_id":"269308212","term_text":"NM_028133"}} NM_028133 AAGTTACACGGAGGGGTCCT GGCTGGACTTCATGTGGATT SOD1 {"type":"entrez-nucleotide","attrs":{"text":"BC002066","term_id":"12805214","term_text":"BC002066"}} BC002066 GAGACCTGGGCAATGTGACT TTGTTTCTCATGGACCACCA SOD2 {"type":"entrez-nucleotide","attrs":{"text":"BC018173","term_id":"17390378","term_text":"BC018173"}} BC018173 AACTCAGGTCGCTCTTCAGC GCTTGATAGCCTCCAGCAAC Catalase {"type":"entrez-nucleotide","attrs":{"text":"NM_009804","term_id":"157951740","term_text":"NM_009804"}} NM_009804 CCAGTTGGCAAAGTGGTTTT GCCCTGAAGCTTTTTGTCAG GST-μ NM_0010358 CACAAGATCACCCAGAGCAA GGTGTCCATGACCTGGTTCT Open in a separate window PRIMERS FOR MICROARRAY PRODUCTION AND RT-PCR Microarray Experimental Design The microarray experiments were performed using a “loop” design ( 27 ) ( ).
Reverse Transcription Polymerase Chain Reaction:Article Title: Gene Expression Profiling of the Hypoxia Signaling Pathway in Hypoxia-Inducible Factor 1α Null Mouse Embryonic Fibroblasts
Article Snippet: The full 15K set was printed on TelechemTM Superaldehyde slides (Telechem International Inc., Sunnyvale, CA) using an OmniGridTM robot (GenMachines ® , San Carlos, CA) with Chipmaker 2 microarray pins (Telechem International Inc). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Gene Accession # Forward Reverse M13 CTGCAAGGCGATTAAGTTGGGTAAC CTGCAAGGCGATTAAGTTGGGTAAC HPRT {"type":"entrez-nucleotide","attrs":{"text":"NM_013556","term_id":"96975137","term_text":"NM_013556"}} NM_013556 AAGCCTAAGATGAGCGCAAG TTACTAGGCAGATGGCCACA GAPDH {"type":"entrez-nucleotide","attrs":{"text":"M32599","term_id":"193423","term_text":"M32599"}} M32599 ACCCAGAAGACTGTGGATGG TTCAGCTCTGGGATGACCTT P4H {"type":"entrez-nucleotide","attrs":{"text":"NM_011031","term_id":"226874875","term_text":"NM_011031"}} NM_011031 CTGTTGTGGCCCGAGTAAAT GAAGTCGAAGTGCGGTTCAT PHD1 {"type":"entrez-nucleotide","attrs":{"text":"AF453879","term_id":"18182545","term_text":"AF453879"}} AF453879 GGAACCCACATGAGGTGAAG AACACCTTTCTGTCCCGATG PHD2 {"type":"entrez-nucleotide","attrs":{"text":"XM_134453","term_id":"25071364","term_text":"XM_134453"}} XM_134453 GAAGCTGGGCAACTACAGGA CATGTCACGCATCTTCCATC PHD3 {"type":"entrez-nucleotide","attrs":{"text":"NM_028133","term_id":"269308212","term_text":"NM_028133"}} NM_028133 AAGTTACACGGAGGGGTCCT GGCTGGACTTCATGTGGATT SOD1 {"type":"entrez-nucleotide","attrs":{"text":"BC002066","term_id":"12805214","term_text":"BC002066"}} BC002066 GAGACCTGGGCAATGTGACT TTGTTTCTCATGGACCACCA SOD2 {"type":"entrez-nucleotide","attrs":{"text":"BC018173","term_id":"17390378","term_text":"BC018173"}} BC018173 AACTCAGGTCGCTCTTCAGC GCTTGATAGCCTCCAGCAAC Catalase {"type":"entrez-nucleotide","attrs":{"text":"NM_009804","term_id":"157951740","term_text":"NM_009804"}} NM_009804 CCAGTTGGCAAAGTGGTTTT GCCCTGAAGCTTTTTGTCAG GST-μ NM_0010358 CACAAGATCACCCAGAGCAA GGTGTCCATGACCTGGTTCT Open in a separate window PRIMERS FOR MICROARRAY PRODUCTION AND RT-PCR Microarray Experimental Design The microarray experiments were performed using a “loop” design ( 27 ) ( ).
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